Grid glow discharge: This video shows you the first step in the sample preparation process- how to correctly perform glow discharge of the electron microscopy grids for the MiniTEM system using a grid glow discharge unit equipped with an oil pump.
After you hydrophilize your sample, go to video "2. Sample preparation negative staining":
https://www.youtube.com/watch?v=8HqkcZSsFwA&
Or are you using a fume hood? See video "3. Negative staining in a biosafety cabinet": https://www.youtube.com/watch?v=a7Py93DUknI
