AlphaFold | Protein Structure Prediction | Mutation Analysis | Step-by-Step Tutorial | English

In this tutorial, we demonstrate how to predict protein 3D structures using AlphaFold through ColabFold. The video provides a complete step-by-step workflow, starting from obtaining a protein sequence from UniProt, predicting the wild-type structure, introducing a mutation, and then predicting the mutant structure. We also explain important structural biology concepts such as protein folding, point mutations, RMSD, and AlphaFold confidence scores like pLDDT. The predicted structures are visualized and compared using molecular visualization tools such as PyMOL to identify structural differences between wild-type and mutant proteins. This tutorial is useful for students and researchers working in bioinformatics, computational biology, structural biology, and protein engineering.