Ames Test

This video is brought to you by the Faculty of Life Sciences at the University of Reutlingen in Germany. The video was created by sixth semester students in the BioMed Laboratory of the Bachelor of Biomedical Sciences program. The students Judith Bohnacker, Sophia Dold, Annika Jany and Jana Staib participated in this video and were supervised by Prof. Dr. Kemkemer and Dipl. Ing. FH Athanasopulu. It contains the following content: The mutagenicity of a chemical compound can be evaluated using the Ames test, a bacterial reverse mutation assay. Mutagenic chemicals cause mutations in the genome, resulting in defective protein synthesis that can have significant effects on an organism, such as causing cancer. The Ames test uses a strain of bacteria in which the mutation determines growth or death. The first step is to plate the mutant bacterial strain, and once it is expanded, the potential mutagen is added. The plate is then incubated and evaluated. If the added substance has mutagenic properties, a reverse mutation occurs and the bacterial strain can now synthesize hormones essential for growth. Many revertant colonies can be counted. Because of its simple principle for detecting mutagenic compounds in food, drugs, or cosmetics, many modified Ames tests have been developed. For more information about the Bachelor of Biomedical Sciences visit the following link: https://www.ls.reutlingen-university.de/de/studium/bachelor-biomedizinische-wissenschaften/ For more information about the BioMED laboratory visit the following link: https://biomed-lab.reutlingen-university.de/home/?L=0 We do not guarantee complete accuracy of the video content