The following set of videos are a basic introduction of how to process your sequencing data from raw .fast5 files through to what programmes to use for downstream analysis such as creating a phylogenetic tree. The tutorials provide information on some programmes and commands that you can use to process your Oxford Nanopore data. For further information and to download the accompanying PDF, please visit https://pandora.tghn.org/sequencing/sequencing-tutorials/
This section covers some of the many programme options you have for analysing your processed ONT sequencing data. It covers:
- Online databases
- Using the EPI2ME agent
- Annotating your genome
- Variant calling
- Identifying antimicrobial resistance genes
- Creating a phylogenetic tree
Apologies for the poor sound quality in parts of the video, presented by @linzyelton4592
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Bioinformatics part 4: Downstream analysis programmes | NatokHD