Confocal Microscopy Explained | Principle, Applications & Limitations

Confocal Microscopy Explained | Principle, Applications & Limitations In this Lecture: 00:00:00 Introduction 00:04:40 In-Focus and Out-Of-Focus Light 00:06:54 Confocal Microscopy overcome limitations of Widefield Fluorescence Microscopy 00:11:55 Difference between Widefield Fluorescence and Confocal Microscopy 00:14:32 Light Source in Widefield Fluorescence and Confocal Microscopy 00:17:00 Use of Pinhole aperture in Confocal Microscopy 00:22:48 Z-stack Imaging 00:26:47 Non-Invasive Optical Sectioning00:31:30 What is Confocal Microscopy 00:33:03 How Confocal Microscopy capture image? 00:40:38 Abbe Equation in Confocal Microscopy 00:45:55 Point Spread Function (PSF) 00:58:30 Applications of Confocal Microscopy Lectures in this series 1. Bright Field and Dark Field Microscope- https://youtu.be/QkteQKGYcCo 2. Phase contrast and DIC Microscope- https://youtu.be/MkXpT4Se1qQ 3. Widefield Fluorescence Microscope: https://youtu.be/-PCXhu8adSM SUBSCRIBE our Channel to stay connected PRESS the BELL icon for all the NOTIFICATIONS LIKE our videos if you really find them helpful Follow us on our social media handles: FACEBOOK PAGE: https://www.facebook.com/ReplicatingMinds INSTAGRAM: @replicating_minds LINKEDIN: https://www.linkedin.com/in/sanjay-kumar-22177b131/ ____________________LIKE || SHARE || SUBSCRIBE______________________ --------- * Stay Connected and Keep Watching Replicating Minds * ---------- Thank You so much & Good Luck Replicating Minds Sanjay Kumar [email protected] ___________________________ ************ _____________________________