Cytek Aurora Training Part 3: Panel Design Tools

Aurora User Training Spectral Flow Cytometry Extended panel design video: https://youtu.be/rtUn_cTpp8A. Everything in the shorter design tools video is also included in the tutorial video - it is not necessary to watch both. PART 3: Panel Design Tools 0:00 Intro 1:58 What makes a panel "good"? 3:25 How autofluorescence can impact panel design 7:56 What is spillover-spreading error? 18:31 General tips for pairing markers and fluors based on spreading error 20:51 Spectrum Viewer 24:34 Spread matrix 28:43 Panel difficulty levels 31:08 Final notes This is a comprehensive course for researchers who would like to use the Cytek Aurora. This course assumes that researchers are already familiar with conventional flow cytometry. Slides can be downloaded here: https://drive.google.com/file/d/1wF9-Zhc2frmM2kxi3R6q4reOxU_eAXyB/view?usp=sharing More information and resources can be found here: https://voices.uchicago.edu/ucflow/spectral-flow-cytometry/ UChicago Aurora panel design tools: https://voices.uchicago.edu/ucflow/project/cytek-aurora-panel-design/ View the full training course: https://youtube.com/playlist?list=PLNDeR64KSOoVRZxeruf3rDHPER4MEb2M5 Cytometry and Antibody Technology Facility University of Chicago I have no affiliation with Cytek Biosciences