Designing Self-Assembling Protein Nanoparticles

This video covers the basics of self-assembling nanoparticles, relationships between native, evolved, designed and de novo nanoparticles and the intermolecular interactions that dictate their structure. Recommendations for interface design, examples for functional design, integration of Rosetta and ProteinMPNN tools for design workflow, existing libraries generated through docking, and nanoparticle design with RFdiffusion. Video from the Rosetta Commons PPI Workshop (February 2025) Video Instructor: Cyrus Haas (University of Washington) Credits: Instructor: Cyrus Haas Teaching Assistants: Yehlin Cho, Matthew Hvasta, and Amrita Nallathambi RC Leadership and NSF Sponsor Grant PIs: Julia Koehler Leman & Jeffrey Gray RC Education Director: Ashley Vater Videographer: Canyon Florey Rosetta Workshop Participants 00:00 - Introduction 01:04 - Native 10:05 - Evolved 12:42 - Classically designed 26:00 - De novo Designed