In this video, I talked about different RNA-Seq normalization methods - RPKM/FPKM and TPM and demonstrated how to calculate these values from counts.
Leave your thoughts in the comment section below!
Link to some reading material:
1. https://haroldpimentel.wordpress.com/2014/05/08/what-the-fpkm-a-review-rna-seq-expression-units/
2. https://www.rna-seqblog.com/rpkm-fpkm-and-tpm-clearly-explained/
Chapters
0:00 Intro
0:42 Types of Replicates
3:07 Biases (gene length and sequencing depth)
5:12 RPKM/FPKM and TPM normalization
7:16 Calculating RPKM values
11:26 Calculating TPM values
14:04 RPKM/FPKM vs TPM
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Website: https://bioinformagician.org/
Github: https://github.com/kpatel427
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