Direct and indirect flow cytometry video protocol

Flow cytometry is a popular laser-based technology to analyze the characteristics of cells or particles. Watch our flow cytometry video protocol that teaches you how to prepare and stain your cells for flow cytometry analysis. View all our video protocols with written procedures at: http://www.abcam.com/tag/video-protocols Connect with us Visit us on: https://www.abcam.com​​ Facebook: https://en-gb.facebook.com/Abcam/​​ Twitter: https://twitter.com/abcam​​ Instagram: https://instagram.com/abcamlive Timestamps: 00:00 Introduction 00:13 What you will need 00:41Titration (optional) 01:00 Preparing a primary antibody solution 01:15 Re-suspending cells and measuring cell viability 01:48 Centrifugation 02:03 Blocking step 02:37 Staining with primary antibody 03:51 Secondary antibody step (optional) 04:55 Checking for live/dead cells 05:16 Storing the cell suspension