Learn how to use FIJI (ImageJ) to do deconvolution on confocal images acquired in 3D or as optical sections (z stacks). This tutorial uses the PSF Generator to model the imaging process of a microscope via a theoretical point spread function (PSF) and the DeconvolutionLab2 plugin to help restore the effective specimen representation (maximize observed resolution and signal) for 3D confocal microscopy images.
You can download the plugins from these sites:
PSF Generator: http://bigwww.epfl.ch/algorithms/psfgenerator/
DeconvolutionLab2: http://bigwww.epfl.ch/deconvolution/deconvolutionlab2/
Both plugins were developed at EPFL - Biomedical Imaging Group (BIG).
SUBSCRIBE to have first access to new video tutorials: https://www.youtube.com/channel/UCFKPbB-h1GbuOG9gwD1tXEA
