How to program HIFLOW pumps with PNet software for gradient making?

Two HIFLOW peristaltic pumps are controlled by PNet PC control software to generate density gradients in automated purification of isolated human islets. More information at http://www.peristalticpump.info/single-post/density-gradient-based-islet-separation-using-computer-controlled-LAMBDA-pumps PNet software controlled peristaltic pumps need instructions for dosing high and low density solutions at specific time points. This precise dosing ensures consistent gradient production. Video credits: http://nordicislets.medscinet.com/en/islet-isolation/tissue-purification.aspx