In this video the process of fluorescence is explained as well as what fluorescence anisotropy actually is. Then a calculation to determine binding affinity is shown.
Link to reference textbook: https://www.amazon.com/Principles-Fluorescence-Spectroscopy-Joseph-Lakowicz/dp/0387312781/ref=sr_1_1?dchild=1&keywords=Principles+of+Fluorescence+Spectroscopy&qid=1608477012&sr=8-1
