Lattice Light Sheet Microscope Practical Demonstration by Mathieu Ducros (Bordeaux Imaging Center), Jean Salamero, Ludovic Leconte and Cesar Valades-Cruz (Curie Institut)
00:00 Introduction
01:02 Lattice Light-Sheet Microscopy (LLSM) Principle
06:40 LLSM homemade setup at Bordeaux Imaging Center (BIC)
08:13 LLSM from 3i at Curie Institute
09:45 Comparative performances
11:38 Sample preparation and mounting at Curie Institute
14:48 Brain slices mounting at BIC
17:40 Calibration Steps
19:55 Example of data acquisition, deskewing and deconvolution
20:15 Example of images acquisition and analysis at Curie Institute
21:44 Photomanipulation path at BIC
25:18 Image processing
27:06 Data managment
28:50 LLSM summary
