Liposome preparation methods and characterization

Physical Dispersion Methods for Liposomes Preparation Hand Shaken and Non Shaken Methods with Processing techniques to optimize liposomes Solvent solvent dispersion for single emulsion to multiple emulsion and reverse phase evaporation methods Ethanol (Protic solvent) and Ether (Lewis solvent) Injection Methods Detergent Solubilization liposomes Removal of surfactants from liposomes Phospholipid analysis (Barlett Assay) Cholesterol analysis Biological Characterization (Sterility, Pyrogenicity, Toxicity)