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Make Fluorescein

19.3K views
Jan 8, 2012
6:08

In this video we synthesize Fluorescein. The procedure is very simple... Mix 0.1 gram each of phthalic anhydride and resorcinol (I bought both at chemkitdepot.com) in a test tube and add 4-5 drops conc. sulfuric acid. Heat gently for 2 minutes. Allow to cool, add 5cc water, and make basic with sodium hydroxide. Mix well and filter off any solids. Store in an air tight bottle.

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Make Fluorescein | NatokHD