This video describes the sample preparation procedures for microbial identification using MALDI-TOF MS and MicrobialTrack.
It covers all major steps, including preparation of reagents and equipment, matrix solution preparation, and preparation of test and calibration samples. Important points and notes for each step are also provided.
0:00 Start
0:14 Items to be prepared
0:44 Step 1: Preparing Matrix Solution
1:08 Step 2: Preparing Specimen
1:32 Step 2: 1. Direct smear method
2:11 Step 2: 2. On-plate formic acid extraction method
3:04 Step 2: 3. Ethanol wash formic acid / acetonitrile extraction method
6:44 Step 2: 4. Ethanol wash heated formic acid / acetomitrile extraction method
10:33 Step 3: Preparing Calibration Samples
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#MALDI #MicrobialIdentification #SamplePreparation #Matrix #Calibration #MicrobialTrack #Shimadzu
