Next Generation Sequencing Applications

Presented here is a brief explanation of how Next Generation Sequencing has become central to Biotechnology research. Towards the end, I also discuss the prerequisites for analyzing NGS data. This lecture is the second part of the webinar I gave at the Hindu College, University of Delhi in 2020. #NGS #Next_Generation_Sequencing #Illumina #Ion_torrent #Nanopore #SMRT #Genomes #Sequencing #DNASeq #RNASeq #Metagenomics #Pharmacogenomics #lets_grow_together #vipins_e_classroom 0:00 Introduction 0:13 NGS Broad Applications 1:14 Key features of NGS 2:18 Exome Sequencing 3:48 NGS based diagnostics - Targeted gene sequencing 7:10 Metagenomics - 16S RNA typing 7:38 Microbiome project 8:40 Pharmacogenomics 8:55 Purine Analogs: A Case Study in Pharmacogenetics 10:38 TPMT Variants and effect on drug dosage 12:07 From conventional to individualized medicine based on TPMT poly 13:57 Genome Sequencing Milestones 14:54 Largest genome ever sequenced 15:27 FASTQ - NGS FILE FORMAT 16:00 Short read aligners 16:41 References 17:01 Desired skill set