Welcome to this demonstration video on Pairwise Distance Analysis — a fundamental step in DNA sequence comparison and phylogenetic studies.
In this session, we’ll explore how to calculate the genetic distance between sequences using the p-distance method, which simply looks at the proportion of nucleotide differences between pairs of sequences. This technique helps us understand how closely or distantly related different organisms are, based on their genetic material.
You’ll learn how to:
Import aligned DNA sequences
Generate a pairwise distance matrix
Interpret the values to infer evolutionary relationships
Whether you're working on species identification, phylogenetic tree building, or barcoding projects — understanding pairwise distance is key to making meaningful conclusions from your DNA data.
Let’s get started!