Part 3: Multiple sequence alignment

Go deeper into the mechanics of pairwise and multiple sequence alignment algorithms and how they are used in phylogenetics. Professor Michael Charleston explains: [0:00] What ‘alignment’ really means and why it is useful for phylogenetics [6:22] What makes a good alignment [12:15] How pairwise sequence alignment and dynamic programming works [33:47] How the multiple sequence alignment algorithms behind Clustal, Muscle, MAFFT and other programs work [40:00] Viewing the multiple sequence alignment of the Anolis sequences with SeaView and correcting errors. This video is part of the tutorial Phylogenetics: back to basics. You can find the full tutorial on the Galaxy Training Network: https://training.galaxyproject.org/training-material/topics/evolution/tutorials/abc_intro_phylo/tutorial.html This tutorial demonstrates the principles behind a variety of methods used to estimate phylogenetic trees from aligned sequence data or distance data. It was developed by Professor Michael Charleston from the University of Tasmania in collaboration with Australian BioCommons. Captions are automatically generated using Otter.ai and edited by the Australian BioCommons team.