( http://www.abnova.com ) - Midiprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 15-25 ml of LB broth and the expected DNA yield is 100-350 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, megaprep, and miniprep. More videos at Abnova http://www.abnova.com