Plasmid DNA Isolation - Improved Yield, No Kit

Cheap, high yield plasmid DNA isolation procedure based on an improved alkaline lysis. This plasmid DNA isolation can produce 43 micrograms of high quality plasmid from a 1.7 mL culture. As a bonus it is fast and costs about 5 cents. No expensive kit is required, making this procedure perfect for labs on a budget. A print protocol can be downloaded here: https://patharkar.com/2019/03/plasmid-dna-isolation-improved-yield-no-kit