Let's find out how much genes changed in abundance (TPMs) during the treatment of Dox. We will also learn a key skill of pivoting data tables in R. This allows us to group by conditions and get average and standard deviation (error) from our replicates across time ! It's a bit tricky to get your head around at first (at least it was/still is for me). But absolutely a key concept to learn in R and we get to find new results too !
*** Oh and extra bonus material on how to make a function to bundle all the skills learned in this lesson. *** See next video and at the bottom of the RMD file on github.
All data needed for this calls can be downloaded here:
https://rinnformatics.colorado.edu/mouse_rnaseq/
John Rinn Github for this class:
https://github.com/boulderrinnlab/MASTER_CLASS/blob/main/lessons/07_visualization_figures/04_TPM_plots.Rmd
John Rinn CGL Website for this class:
https://www.lncrna.io/cgl-level-7
-TPM Plots
John Rinn BioFrontiers:
https://www.colorado.edu/biofrontiers/john-rinn
John Rinn Lab:
lncRNA.io
John Rinn Teaching:
lncrna.io/teaching
John Rinn University of Colorado Boulder Biochemistry:
https://www.colorado.edu/biochemistry/john-rinn
John Rinn Linkedin:
https://www.linkedin.com/in/john-rinn/
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Plotting TPM values - while learning how to melt data (John Rinn Computational Genomics Lab) | NatokHD