Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

Reference: https://app.jove.com/v/3998/polymerase-chain-reaction-basic-protocol-plus-troubleshooting Ample quantities of a particular DNA segment (i.e., an amplicon) can be produced using the potent amplification method known as polymerase chain reaction (PCR) from a relatively small quantity of starting material, such as a DNA template or target sequence. This protocol explains the fundamentals of PCR, offers a method that will amplify the majority of target sequences, and offers suggestions for improving a reaction.