Light Microscopy - Fundamental Principles - Refractive Index and Sample Preparation
An understanding of refractive index is fundamental to light microscopy. This talk explains what refractive index is and how it can affect resolution. I discuss how refractive index relates to aspects of your light microscopy imaging experiments such as objective lens choice and sample preparation. The correct choices of objective and sample mounting media will lead to improved brightness and resolution in your resulting data.
Links to related resources:
https://www.youtube.com/c/Microcourses
https://www.ibiology.org/online-biology-courses/microscopy-series/microscopy-series-table-contents/
