Williams Lab | Bates College | Instructional Video Series | May 2015
0:40 – Step 1: Homogenize embryos in Stat60
1:30 – Step 2: Incubate embryos at room temp for 5–20 mins
1:35 – Step 3: Add chloroform to the solution and mix
1:59 – Step 4: Centrifuge the samples and pipet the upper phase
2:37 – Step 5: Add RNA binding buffer
2:46 – Step 6: Add ethanol
3:03 – Step 7: Transfer the mixture to the Zymo columns and centrifuge
3:36 – Step 8: Add RNA prep buffer and centrifuge
3:49 – Step 9: Add RNA wash buffer and centrifuge
4:01 – Step 10: Centrifuge the column and transfer to a new tube
4:10 – Step 11: Add mobio water to the column and centrifuge
