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SAXS: sample and buffer preparation

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Mar 22, 2021
48:46

How to prepare your buffer and sample for a successful solution SAXS experiment - a practical guide by Melissa Gräwert 0:00 The small angle X-ray scattering experiment 1:24 Sample requirements 14:26 Sample characterization 16:40 Buffer preparation 21:58 SEC-SAXS mode 26:35 Performing an experiment: 5 cases 27:12 1: the Relaxed Scientist (wrong concentration) 30:54 2: the Lazy Scientist (concentration effects) 33:17 3: the Hasty Scientist (incorrect buffer subtraction) 37:29 4: the Super Scientist (polydisperse sample) 41:11 5: the Brilliant Scientist (radiation damage) 44:12 Summary

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SAXS: sample and buffer preparation | NatokHD