Sequence Development and Execution in quick start Empower 3 Part 2/2

​Step-by-Step Sequence Run Procedure ​The sequence in Waters software is generally referred to as a Sample Set. Here is the standard workflow to get your run started: ​1. System Equilibration ​Before starting the sequence, ensure your system is ready. ​Purge: Flush the pump to remove air bubbles. ​Prime: Ensure the needle wash and seal wash are flowing. ​Condition: Run the mobile phase through the column at the starting gradient until the pressure and baseline are stable. ​2. Create the Sample Set Method ​In the Empower "Run Samples" window, navigate to the Sample Set Method tab. ​Insert Rows: Add rows for your Vials, Standards, and Samples. ​Function: Define each row (e.g., Inject Standards, Inject Samples, or Clear Calibration). ​Vial Position: Enter the exact location of your vials in the autosampler tray. ​Injection Volume: Specify how many \mu L to inject. ​Method Set: Select the specific Method Set (which includes the Instrument Method and Processing Method) you created for this analysis. ​3. Set Up "Run and Report" ​Decide how you want the software to behave during the run: ​Run Only: Acquires raw data for manual processing later. ​Run and Report: Automatically processes the data and prints a report after each injection. ​4. Check the "Run Time" and "Samples" ​Total Time: Ensure your run time is long enough for all peaks to elute and the column to re-equilibrate. ​Vial Check: Physically verify that the vials in the tray match the positions entered in your Sample Set. ​5. Start the Run ​Click the Green Run Button (Load/Run). ​Select Run Sample Set. ​Monitor the System Monitor to ensure the first injection occurs and the pressure remains within safe limits.