Setup Tutorial for Recirculation Perfusion System w. iFlow Microfluidic Controller for cell culture

Recirculating Perfusion System -Cell Culture under Flow Microfluidic controller and applications. https://www.precigenome.com/recirculating-perfusion-system Combining our PG-MF controller with a 3-way/2-position rotary valve, PreciGenome provides a perfusion system dedicated to cell culture in perfusion chambers with controlled shear force. This fully integrated solution includes all the necessary elements (hardware and software) to create a continuous and recirculated (unidirectional or bidirectional) flow and controlled flow rate (shear force) applied onto cells. ​ PG-MFC controller is used to provide pressure to pump reagent from reservoir 1 through perfusion chamber to reservoir 2 through the valve. Combining PG-MFC pressure controller with the rotary valve ( extra two 3-way valves required for light version controller), the system allows flowing buffers in two separate reservoirs back and forth, but still keeps the flow in the microfluidic chip uni-directional.