Learn how to perform spore counting using the Terragene® method, in accordance with ISO 11138-1:2017.
This video features the SCBI Bionova® BT224 as an example.
Please note that specific steps may vary depending on the biological indicator presentation (e.g., Spore Strips, Spore Ampoules, or Conventional SCBIs).
📄 For more information:
Refer to the Technical Note: Terragene Spore Count Protocol
https://terragene.com/wp-content/uploads/NotasTecnicas/TN-04%20Spore%20count%20Rev.13.pdf
0:00 Intro
0:37 Materials Needed
0:53 Spore Carrier Removal
1:11 Initial Suspension Preparation
1:59 Spore Activation: Physical Treatment
2:26 Spore Activation: Thermal Treatment
2:54 Spore Activation: Cold Bath
3:10 Serial Dilution Preparation
4:30 Plating on Culture Media
5:30 Incubation
5:56 Population Counting and Calculation
