In microscopy, Gaussian noise arises from many sources including electronic components such as detectors and sensors. In addition, salt & pepper noise may also show up due to errors in analog to digital conversion. Therefore, image denoising is one of the primary pre-processing operations that a researcher performs before proceeding with extracting information out of these images.
This tutorial explains Total Variation Filter (TVF) and walks you through the process of writing a couple of lines of code in Python to implement the filter.
References:
https://hal.archives-ouvertes.fr/hal-00437581/document
Code associated with these tutorials can be downloaded from here: https://github.com/bnsreenu/python_for_image_processing_APEER
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Tutorial 36 - Image filtering in python - Total variation filter (TVF) for image denoising | NatokHD