With this video you can learn to put together your own scratch assay analysis with only a few lines of code.
The wound healing or scratch assay is a standard technique for probing collective cell migration in two dimensions. A monolayer of cells are scratched with a pipette tip. The migration of cells into the gap is imaged over several hours using a microscope. The primary information derived is the rate of gap closure, a measure of the speed of the collective motion of the cells.
References:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154238/
Code associated with these tutorials can be downloaded from here: https://github.com/bnsreenu/python_for_image_processing_APEER
