UV-Vis Tutorial | Part 2: Performing a Quantitative Measurement

The second part in a series on how to accurately measure the optical spectra of solutions of nanoparticles using a UV-VIS (UV-Visible) spectroscopy. In this demo, we use an Agilent 8453 spectrophotometer to accurately measure a high concentration solution of nanoComposix's BioPure Silver Nanospheres using an analytical balance. The video discusses: • Optimal absorbance to take UV-Vis measurements • Measurement techniques for performing a dilution • Critical data that needs to be captured in order to accurately perform a quantitative calculation Guidelines for UV-Vis Analysis handbook: http://nanocomposix.com/pages/handbooks Other useful links: • Optical Properties of Silver Nanoparticles: http://nanocomposix.com/pages/silver-nanoparticles-optical-properties • Optical Properties of Gold Nanoparticles: http://nanocomposix.com/pages/gold-nanoparticles-optical-properties • Introduction to Plasmonic Nanomaterials: http://nanocomposix.com/pages/plasmonics-and-nanophotonics • Overview of Nanoparticle Characterization Techniques: http://nanocomposix.com/pages/characterization-techniques Part 1 of this series can be found here: https://youtu.be/wK7ue8Uesbw Let us measure your sample for you! Find out more about our UV-Vis measurement services and other nanoparticle characterization services at nanocomposix.com Thanks for watching! nanoComposix Team [email protected] (858) 565-4227 nanocomposix.com