Link to website with slides used in this presentation, the 3D models I've made for vibratome parts, and my contact info: https://sites.google.com/view/kru-neu...
This video is a presentation in which I elaborate on the issues that can happen when sectioning (slicing) tissue specimens with a vibratome. I also provide various tips to help you avoid these issues.
Edit 7-29-2025: See also my pinned comment on blade choices below.
Time stamps:
0:00 - Intro and Disclaimer about live tissue
1:15 - Common issues with sections acquired by vibratome
8:48 - Mechanism behind the main thick-to-thin section issues, and ways to resolve them
27:07 - Other reasons for tissue artifacts, and solutions for those
33:16 - Optimizing cutting parameters
53:12 - Live tissue prep considerations
1:01:12 - Conclusion
