Webinar: Stereology Question & Answer

Click 'show more' for a list of questions and a time stamp of when each question is answered. Q. For the nucleator probe, how many IUR sections and nuclei do you need to count? 0:50 Q. I have a BIOQUANT system but am interested in your software as an alternative. I do not have a microscope with a motorized stage, so please cover whether and how I could use Stereo navigator with my current microscope.4:17 Q. My collegue and I were wondering if the Optical Fractionator is immune to differential shrinkage? 6:35 Q. How to use the Cavalieri estimate in estimating whole volume in subsequent big coronal sections of the human brain? 8:44 Q. How can I estimate the whole number of fibers in a determined brain region? 13:23 Q. I have a very similar question about blood vessels. How can I accurately estimate the blood vessels density in a region of interest in the brain? 15:48 Q. Which thickness would you recommend for coronal sections?17:55 Q. How do you estimate the number of motor neurons in a spinal cord segment? 22:11 Q. Confocal stereology of small structures, like varicosities. Please elaborate.24:06 Q. OF on confocal stacks: do you have to have the confocal microscope linked live to SI in order to obtain SRS samples or can you do it separately (non live) and then import the images to SI for OF counts? 26:38 Q. What is the best way to determine the optimal magnification of images for stereology? 27:36 Q. Are both top and bottom zones needed? Sometime, if both are used, the section thickness will be too thin and hard to count. Is that OK to use only one zone, for example only top zone? 31:47 Q. 20um is required for neuronal cells, but not other smaller volume cells? 33:49 Q. When using the optical fractionator, do you recommend using the highest possible magnification for counting? 35:20 Q. Does the Stereo investigator program have a function that allow you to estimate the optimum number of cell needed per counting frame and the number of sampling site to avoid over sampling or under sampling? 35:50 Q. What can I do to determine how much my tissue is shrinking after processing? 43:49 Q. We count different regions in the brain of mice and these regions overrides with different size. How can I determine the interval tissue sections? 44:46 Q. How can we define microglia activation using stereology, other than cell count? 47:26 Q. Related to the previous question; Stereo investigator to estimate damaged volume like infarct, optical magnification? 49:18 Q. Could we clarify our question from earlier regarding differential tissue shrinkage; Differential shrinkage meaning "when particle densities are non-uniform through the section". We are not referring to uniform tissue shrinkage. 50:45 Q. How is MBF developing to allow really thick sections as in clarity methods? 52:13