the bumbling biochemist

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Assistant Professor of Biochemistry at Loyola Marymount University trying to make biochemistry fun and accessible for all. I aim for lots of detail without l...

Latest Videos

Walkthrough of my postdoc paper probing how certain antibiotics inhibit mitochondrial ribosomes

Types of professor, tenure track, etc. (more of that hidden curriculum of academia stuff)

ORCiD IDs What those green iD circles are all about and why you should get one & check them out!

The bumbling biochemist turns 10! And she has a birthday wish . . .

Stacking and resolving gels and gel percentages in PAGE gels

Who needs TV when you've got FPLC Watching protein elute (come off from) a SEC column

Bibel lab @ LMU 1 (academic) year birthday party!

Strategically approaching scientific research articles - how I like to set up for a deep dive

Paper deep dive Designing isoleucine-less proteins towards a minimized (19 aa) protein alphabet

What's Journal Club

Practical pipetting tips

Lab tip Use a quick Bradford assay to tell when to move on in your protein column chromatography

An overview of structural biology techniques – crystallography, cryo-EM, NMR, computational methods

Staying organized with lab inventory spreadsheets and logs (especially for protein biochemists)

Annotating electrophoresis gels in Adobe Illustrator

Converting UV280 absorbance to protein concentration - finding conversion values & using on NanoDrop

Scientific research posters – what they are & advice for preparing and presenting them

Batch binding with gravity flow chromatography

Satellite colonies with ampicillin selection

Blue Native PAGE

Biomolecular conjugates

Interpreting SDS-PAGE gels & using them to assess protein purification scheme efficiency & efficacy

Colloidal Coomassie Brilliant Blue stains – the way Instant protein gel stains work

Practical tips for avoiding gel smiling & getting a nice PAGE (SDS-PAGE, urea-PAGE, etc.)

Peeps Ideal Gas Law demo

Book rec My life, my science Pursuing a Cure for Huntington's Disease by Nancy Sabin Wexler

A guide to types of science papers Research articles, review papers, & more

Empirical determination

Conservation of protein sequences conserved vs. conservative (substitutions)

Scientific CV (Curriculum Vitae) advice