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Basic bioinformatics for Oxford Nanopore sequencing data analysis

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Mar 16, 2023
27:02

This presentation, led by Dr John Tembo from HerpeZ, Zambia demonstrates how to basecall using Guppy (processing raw fast5 files into fastq files) on Mac and Windows computers, how to concatenate (combine) these files into a single fastq for downstream processing, and how to use FastQC to analyse the quality of your fastq files. For more information about this workshop, please visit the workshop page: https://pandora.tghn.org/training/tuberculosis-sequencing-using-oxford-nanopore-university-teaching-hospital-zambia/ For more information about the tuberculosis sequencing pipeline, please visit the TGHN hub page: https://pandora.tghn.org/sequencing/tuberculosis-sequencing-pipeline/ For image credits and links from the slides, please email [email protected]

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Basic bioinformatics for Oxford Nanopore sequencing data analysis | NatokHD