Cleaning a reverse-phase HPLC column doesn’t have to be complicated. In this step-by-step guide, Dr. Lee Polite explains how to flush out salts, buffers, and sticky non-polar residues using simple solvent changes. You’ll learn exactly when to use water-rich mobile phase, when to switch to 100% organic, and how to keep your columns running longer.
Prefer to read: https://axionlabs.com/chromatography-training/clean-reverse-phase-hplc-column/
👉 Related Videos:
How to Clean a C18 Column
– extra details on cleaning a dirty or clogged frit
https://axionlabs.com/chromatography-training/how-to-clean-a-c18-column/
HPLC Demystified Part 1
– a refresher on why separation depends so much on polarity
https://www.youtube.com/watch?v=SV1-r_58ERQ
How to test an HPLC column
-check efficiency and decide whether cleaning helped
https://axionlabs.com/chromatography-training/how-to-test-an-lc-column/
📌 Topics Covered:
Why columns get dirty (salts, buffers, sample oils)
Step 1: Water flush to remove polar contaminants
Step 2: Organic flush to remove non-polar residues
Shortened cleaning protocol if you’re in a rush
What cleaning can’t fix (physical column damage)
🎓 Want hands-on training? Join us at Axion Labs
in Chicago for 5-day chromatography bootcamps in HPLC and GC.
https://axionlabs.com/lc-gc-bootcamp/
Hashtags:
#HPLC #Chromatography #LabTips #HPLCTraining #ReversePhase #AnalyticalChemistry #HPLCColumns
