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File Formats #3: POD5

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Aug 28, 2025
4:52

In this video, I explain the POD5 file format — a standard for storing raw signal data from Oxford Nanopore sequencing instruments, used for basecalling and modification analysis. 📄 Learn More 🔗 Format → https://pod5-file-format.readthedocs.io/en/0.1.21/SPECIFICATION.html 🔗 Design → https://pod5-file-format.readthedocs.io/en/0.1.21/DESIGN.html 🔗 Python toolkit → https://pod5-file-format.readthedocs.io/en/0.1.21/docs/api.html 💻 Software and Documentation 🔗 Dorado software → https://github.com/nanoporetech/dorado 🔗 Dorado docs → https://software-docs.nanoporetech.com/dorado/latest/ 💾 Data Repositories 🔗 Singapore Nanopore Expression Data Set (SGNex) → https://registry.opendata.aws/sgnex/ 🔗 1KG-ONT-VIENNA panel → https://registry.opendata.aws/1kg-ont-vienna/ 📺 More Genomics & Bioinformatics Videos 👉 Subscribe for tutorials, data analysis guides, and sequencing deep dives!

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File Formats #3: POD5 | NatokHD