Pyrosequencing

Hello Dear learners In this lecture we will discuss about Pyrosequencing. It is a DNA sequencing method that was widely used in research and clinical applications. It has been largely replaced by newer sequencing technologies like Illumina's sequencing-by-synthesis (SBS) technology. However, it remains an important historical method in the field of genomics. Pyrosequencing involves the real-time detection of nucleotide incorporation during DNA synthesis. It is a sequencing-by-synthesis method that does not require labeled nucleotides. Instead, it uses a series of enzymatic reactions to measure the release of inorganic pyrophosphate (PPi) when a nucleotide is added to a growing DNA strand. The release of PPi leads to the production of light, which is quantified to determine the sequence of the DNA template. Chapters : 0:00-4:05 Introduction 4:06-8:02 enzymes involved in pyrosequencing 8:03-11:31 chromatogram 11:32-12:53 types of pyrosequencing 12:54- 20:32 454 DNA sequencing 20:33-23:11 Liquid Liquid pyrosequencing 23:12-25:00 Difference between solid and liquid pyrosequencing 25:00-25:22 Reference and Thanks #DNAsequencing #Pyrosequencing #Nucleotide incorporation #Real-time sequencing #Enzymatic reactions #Luciferase #ATPsulfurylase #Apyrase #Pyrophosphate #Primerannealing #DNApolymerase #Templatepreparation #Highthroughputsequencing #Nextgenerationsequencing #Sequencing-by-synthesis (SBS) #Illumina sequencing #DNAtemplate #ngs #thelearningboosters #csirnet #biotechnology #DNAsequeningMethods