Bioinformatics - Understanding Trimmomatic

In this video I talk about the fastq quality trimming program that we previously installed and used called Trimmomatic. This tool is super easy to use and here I wanted to talk about what the different flags that we are giving Trimmomatic tell it to do. All but the palindrome part of the IlluminaClip is fairly straight forward (I have a hard time wrapping my head around this). If you find this these videos helpful please like and subscribe! It really helps me identify what others find useful! Thank you! Other tools: Btrim: A Fast, Lightweight Adapter and Quality Trimming Program for Next-Generation Sequencing Technologieshttps://pubmed.ncbi.nlm.nih.gov/21651976/ Skewer: a fast and accurate adapter trimmer for next-generation sequencing paired-end reads https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-15-182 fastp: an ultra-fast all-in-one FASTQ preprocessor https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6129281/ Trimmomatic: A Flexible Trimmer for Illumina Sequence Data https://pubmed.ncbi.nlm.nih.gov/24695404/ Not a fan of doing plugs for "Like, Share, and Subscribe" but if you could, I would greatly appreciate it. Thanks! Project Github: https://github.com/ACSoupir/Bioinformatics_YouTube I'm not an expert in bioinformatics, and have only really done basic bacterial genome assembly, some RNA-seq, and a microbiome analysis. There may be better ways of doing things but this is how I do it. In this case this is my best interpretation of the Trimmomatic tool's manual. These video's are learning experiences for everyone. Image at the beginning on the bottom left is modified from AllGenetics.EU. Please consider contributing to my Patreon where I may do merch and gather ideas for future content: https://www.patreon.com/AlexSoupir